PPI.bio Beta

How-To Guide for Proteome-Wide PPI Inference

Using the online ppi.bio interface, users are capable of making inferences between:

  1. a user-defined protein and
  2. all the proteins of a user-specified organism.

Proteome-Wide Diagram

Fill out the "Proteome-Wide Prediction" form

To begin, visit the "Proteome-wide Prediction" page, where you'll be presented with a form asking you for:

  1. The organism whose proteome to use. Interactions between your submitted amino acid sequence and the proteins of this organism will be computed.
  2. The inference model to use to infer PPIs. Currently, RAPPPID and INTREPPPID are supported.
  3. The amino acid sequence of a protein whose interactions you're interesting on inferring. PPIs between the protein encoded by the user-inputted amino acid sequence and the proteome proteins will be inferred.
  4. Hit "Predict Interactions" to start inferring.

Proteome-Wide Submission Form Screenshot

Your task will be added to a queue. Once accepted, a progress bar will appear which shows how your job is going.

Interpreting the Results

Check-out the "Interpreting Results from Proteome-Wide PPI Inference"